新型冠状病毒及其检测方法研究进展A review of severe acute respiratory syndrome coronavirus 2 and its detecting methods
王越珉,雷喜梅,邬丽,伍义行,叶子弘,俞晓平
摘要(Abstract)：
目的:概述新型冠状病毒(SARS-CoV-2,severe acute respiratory syndrome coronavirus 2)病原特征及检测方法的研究现状与发展趋势。方法:总结了SARS-CoV-2的病原特征,分析了病毒分离鉴定、核酸检测和蛋白检测等方法的当前进展。结果:SARS-CoV-2是β属冠状病毒的新成员,具有较强的传染性和隐蔽性;病毒分离鉴定是感染综合诊断的重要依据,但在临床诊断的直接应用中受限;核酸检测特异性强、灵敏性高,但目前假阴性结果频发;蛋白检测可用于疑似病例、无症状病例检测等方面,但质控不足。结论:未来SARS-CoV-2检测技术的研发不仅要注重病毒检测技术的创新,更应重视核酸或蛋白标准样品的研制及全病程监测技术体系的完善,为病毒检测结果的溯源、检测试剂的质量控制、患者的病程监测和抗SARS-CoV-2药物的研发提供保障。
关键词(KeyWords)： 新型冠状病毒;病毒特征;2019冠状病毒病;核酸检测;蛋白检测;检测方法
基金项目(Foundation): 国家重点研发计划项目(No.2017YFF0210200)
作者(Author): 王越珉,雷喜梅,邬丽,伍义行,叶子弘,俞晓平
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