2022 01 v.33;No.105 100-105
不同发育历期褐飞虱脂肪体与肠道内共生菌数量的变化
基金项目(Foundation):
国家自然科学基金项目(No.31401793,3164001);;
浙江省重点研发计划项目(No.2017C32006,2019C02015,2022C02047)
邮箱(Email):
stxp@cjlu.edu.cn;
DOI:
中文作者单位:
中国计量大学生命科学学院浙江省生物计量及检验检疫技术重点实验室;
摘要(Abstract):
目的:褐飞虱(Nilaparvata lugens St?l)脂肪体和肠道内存在大量共生菌，这些共生菌与褐飞虱相互依存相互影响。本研究建立了褐飞虱共生菌的数量测定方法，为揭示共生菌在昆虫体内的功能奠定基础。方法:建立荧光定量PCR反应检测体系，检测不同发育历期褐飞虱脂肪体和肠道内共生菌数量的变化。结果:5龄若虫至羽化后2 d,雌、雄虫肠道共生菌数量显著减少，至羽化6 d,雌虫肠道共生菌数量又有显著增加；3龄至5龄，若虫脂肪体共生菌的数量显著减少；5龄若虫至羽化后2 d,雌虫脂肪体共生细菌数量无显著变化，共生真菌数量显著增加，至羽化后6 d,共生细菌数量显著增加，共生真菌显著减少；雄虫共生菌的变化趋势与雌虫的共生真菌相似。结论:本研究明确了不同发育历期褐飞虱肠道与脂肪体内共生菌数量的变化规律，为后续进一步阐明褐飞虱和共生菌之间的关系提供科学依据。
关键词(KeyWords):
褐飞虱;;肠道;;脂肪体;;共生菌;;荧光定量PCR
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参考文献
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[14] 吕仲贤，俞晓平，陈建明，等.共生菌对褐飞虱生长发育和生殖的影响[J].植物保护学报，2001,28(3):193-197.LV Z X,YU X P,CHEN J M,et al.The effect of endosymbiote on the development and reproduction of brown planthopper Nilaparvata lugens St?l[J].Journal of Plant Protection,2001,28(3):193-197.
[15] NODA H,NAKASHIMA N,KOIZUMI M.Phylogenetic position of yeast-like symbiotes or rice planthoppers based on partial 18S rDNA sequences[J].Insect Biochemistry and Molecular Biology,1995,25:639-646.
[2] BAO Y Y,ZHANG C X.Recent advances in molecular biology research of a rice pest,the brown planthopper[J].Journal of Integrative Agriculture,2019,18(4):18-30.
[3] SHENTU X P,LI D T,XU J F,et al.Effects of fungicides on the yeast-like symbiotes and their host,Nilaparvata lugens st?l(Hemiptera:Delphacidae)[J].Pesticide Biochemistry and Physiology,2016,128(10):16-21.
[4] 吕仲贤，俞晓平，陈建明，等.共生菌在褐飞虱致害性变化中的作用[J].昆虫学报，2001,44(2):197-204.LV Z X,YU X P,CHEN J M,et al.Role of endosymbiote in virulence change of the brown planthopper to rice resistant varieties[J].Acta Entomologica Sinica,2001,44(2):197-204
[5] 王天召，王正亮，朱杭锋，等.基于高通量测序的褐飞虱肠道微生物多样性分析[J].昆虫学报，2019,62(3):51-61.WANG T Z,WANG Z L,ZHU H F,et al.Analysis of the gut microbial diversity of the brown planthopper,Nilaparvata lugens(Hemiptera:Delphacidae) by high-throughput sequencing[J].Acta Entomologica Sinica,2019,62(3):51-61.
[6] 何林懋，申屠旭萍，李丹婷，等.杀菌剂与吡虫啉混合配剂对褐飞虱种群增长的影响[J].中国生物防治学报，2018,34(2):280-286.HE L M,SHENTU X P,LI D T,et al.Effects of fungicide/insecticide mixtures on population growth of brown planthopper,Nilaparvata lugens St?l(Hemiptera:Delphacidae)[J].Chinese Journal of Biological Control,2018,34(2):280-286.
[7] 侯云.褐飞虱体内类酵母共生菌的多样性及其在不同虫龄和不同抗性水稻上的变化规律[D].杭州：中国计量大学，2013:26-40.Hou Y.Diversity of Yeast-Llike Symbiosionts in the Brown Planthopper,Nlaparvata lugens and Variation Rule in Different Resistance Rice and Insect Age[D].Hangzhou:China Jiliang University,2013:26-40.
[8] XUE J,ZHOU X,ZHANG C X,et al.Genomes of the rice pest brown planthopper and its endosymbionts reveal complex complementary contributions for host adaptation[J].Genome Biology,2014,15(12):521.
[9] JU J F,BING X L,ZHAO D S,et al.Wolbachia supplement biotin and riboflavin to enhance reproductionin planthoppers[J].The International Society for Microbial Ecology Journal,2019,14:676-687.
[10] WETZEL J M,OHNISHI M,FUJITA T,et al.Diversity in steroidogenesis of symbiotic microorganisms from planthoppers[J].Journal of Chemical Ecology,1992,18(11):2083-2094.
[11] HONGOH Y,ISHIKAWA H .Uric acid as a nitrogen resource for the brown planthopper,Nilaparvata lugens:Studies with synthetic diets and aposymbiotic insects[J].Zoological Science,1997,14(4):581-586.
[12] 傅强，张志涛，胡萃，等.高温处理后褐飞虱体内共生酵母菌和氨基酸需求的变化[J].昆虫学报，2001(4):534-540.FU Q,ZHANG Z T,HU C,et al.The effects of high temperature on both yeast-like symbionts and amino acid requirements of Nilaparvata lugens[J].Acta Entomologica Sinica,2001(4):534-540.
[13] CHENG D J,HOU R F.Histological observations on transovarial transmission of a yeast-like symbiote in Nilaparvata lugens St?l (Homoptera,Delphacidae)[J].Tissue and Cell,2001,33(3):273-279.
[14] 吕仲贤，俞晓平，陈建明，等.共生菌对褐飞虱生长发育和生殖的影响[J].植物保护学报，2001,28(3):193-197.LV Z X,YU X P,CHEN J M,et al.The effect of endosymbiote on the development and reproduction of brown planthopper Nilaparvata lugens St?l[J].Journal of Plant Protection,2001,28(3):193-197.
[15] NODA H,NAKASHIMA N,KOIZUMI M.Phylogenetic position of yeast-like symbiotes or rice planthoppers based on partial 18S rDNA sequences[J].Insect Biochemistry and Molecular Biology,1995,25:639-646.
基本信息:
DOI：
中图分类号:S435.112.3
引用信息:
[1]熊振泽,史嘉腾,宋阳等.不同发育历期褐飞虱脂肪体与肠道内共生菌数量的变化[J].中国计量大学学报,2022,33(01):100-105.
基金信息:
国家自然科学基金项目(No.31401793,3164001);; 浙江省重点研发计划项目(No.2017C32006,2019C02015,2022C02047)
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