2023 01 v.34;No.109 44-50
基于DNA条形码的稻蓟马、稻叶蝉和稻飞虱种类鉴别
基金项目(Foundation):
国家自然科学基金项目(NO.31972347,U21A20223);;
浙江省属高校基本科研业务费专项项目(NO.2022YW82)
邮箱(Email):
wzl@cjlu.edu.cn;yxp@cjlu.edu.cn;
DOI:
中文作者单位:
中国计量大学生命科学学院浙江省生物计量及检验检疫技术重点实验室;
摘要(Abstract):
目的:研究DNA条形码技术在稻田小型害虫稻蓟马、稻叶蝉和稻飞虱种类鉴别中的适用性。方法:利用DNA条形码的通用引物对稻田3种稻蓟马、5种稻叶蝉和3种稻飞虱的线粒体细胞色素C氧化酶亚基I(Cytochrome c oxidase subunit I, COI)基因序列进行扩增、测序和分析，以Kimura-2-parameter (K2P)模型计算种间和种内遗传距离，并以邻接法(Neighbor-Joining, NJ)构建系统发育树。结果:通过PCR扩增测序结合GenBank公共序列筛选获得11种水稻害虫COI序列共34条，其序列长度位于646～658 bp之间，且碱基组成存在明显的A+T碱基偏向性。K2P遗传距离分析结果显示，11种水稻害虫的种内和种间平均遗传距离分别为0.002和0.326,最大种内和最小种间遗传距离分别为0.004和0.091,种内与种间遗传距离之间存在显著的条形码间隙(barcode gap)。系统发育分析结果显示，NJ进化树中同一物种昆虫聚集于同一进化分支，聚类分析与形态学鉴定结果一致。结论:基于COI基因的DNA条形码技术可以准确鉴别我国水稻上三类小型害虫——稻蓟马、稻叶蝉和稻飞虱。
关键词(KeyWords):
稻蓟马;;稻叶蝉;;稻飞虱;;DNA条形码;;COI序列;;物种鉴定
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参考文献
[1] TANG T,LIU X Y,WANG P,et al.Thiamethoxam seed treatment for control of rice thrips (Chloethrips oryzae) and its effects on the growth and yield of rice (Oryza sativa)[J].Crop Protection,2017,98:136-142.
[2] ZHONG L K,LI H X,YU X F,et al.Complete mitochondrial genome sequence of Cicadella viridis (Hemiptera:Cicadellidae:Cicadellinae)[J].Mitochondrial DNA Part B,2019,4(1):1287-1288.
[3] ZHANG H G,HE B B,XING J,et al.Spatial and temporal patterns of rice planthopper populations in South and Southwest China[J].Computers and Electronics in Agriculture,2022,194:106750(1-12).
[4] ARDESTANI M M.A brief overview of the rice pests and their natural enemies in some rice-growing Asian countries[J].Russian Agricultural Sciences,2020,46(4):359-364.
[5] SCHINDEL D E,MILLER S E.DNA barcoding a useful tool for taxonomists[J].Nature,2005,435:1-17.
[6] BOLSHAKOV V V,PROKIN A A.Karyotype and COI sequences of Chironomus sokolove istomina,Kiknadze et Siirin,1999 (Diptera,Chironomidae) from the bay of Orkhon River,Mongolia[J].Comparative Cytogenetics,2021,15(2):149-157.
[7] SANDRA M V,ELKIN A,JENNY J G,et al.Saving DNA from museum specimens:The success of DNA mini-barcodes in haplotype reconstruction in the genus Anastrepha (Diptera:Tephritidae)[J].Journal of Advanced Research,2019,16:123-134.
[8] LIN X L,MO L D,BU W J,et al.The first comprehensive DNA barcode reference library of Chinese Tanytarsus (Diptera:Chironomidae) for environmental DNA metabarcoding[J].Diversity and Distributions,2021,27(10):1932-1941.
[9] KRESS W J,ERICKSON D L.DNA barcodes:Genes,genomics,and bioinformatics[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(8):2761-2762.
[10] TAANMAN J W.The mitochondrial genome;structure,transcription,translation and replication[J].Biochimica et Biophysica Acta (BBA)-Bioenergetics,1999,1410(2):103-123.
[11] HEBERT P D,RATNASINGHAM S,DEWAARD J R.Barcoding animal life:Cytochrome c oxidase subunit 1 divergences among closely related species[J].Proceedings of the Royal Society of London.Series B:Biological Sciences,2003,270(suppl 1):96-99.
[12] 刘勇，宋毓，李晓宇.基于线粒体COI基因的DNA条形码技术在昆虫分子鉴定中的应用[J].植物检疫，2010,24(2):46-50.LIU Y,SONG Y,LI X Y.Application of DNA barcoding technology based on the mitochondrial COI gene for molecular identification of insects[J].Plant Quarantine,2010,24(2):46-50.
[13] 李青青，李地艳，段焰青，等.DNA条形码在鳞翅目昆虫中的应用[J].生命科学，2010,22(4):307-312.LI Q Q,LI D Y,DUAN Y Q,et al.Application of DNA barcoding in Lepidopteran insects[J].Chinese Bulletin of Life Sciences,2010,22(4):307-312.
[14] 李晓叶，钱路，陈茂华.DNA条形码技术在几种苹果园鳞翅目害虫鉴定中的应用[J].西北农业学报，2015,24(5):141-147.LI X X,QIAN L,CHENG M H.Application of DNA barcoding technology to identification of some Lepidoptera pests in apple orchards[J].Acta Agriculturae Boreali-occidentalis Sinica,2015,24(5):141-147.
[15] 温娟，陈睿，姜立云，等.基于DNA条形码对北京地区蔷薇科花卉上蚜虫的快速鉴定[J].应用昆虫学报，2013,50(1):29-40.WEN J,CHEN R,JIANG L Y,et al.Rapid identification of aphids on flowering plants of Rosaceae in Beijing based on DNA barcoding[J].Chinese Journal of Applied Entomology,2013,50(1):29-40.
[16] 周青松，张同心，于芳，等.DNA条形码在膜翅目昆虫中的应用分析[J].应用昆虫学报，2013,50(1):19-28.ZHOU Q S,ZHANG T X,YU F,et al.Application of DNA barcoding to Hymenopteran taxonomy[J].Chinese Journal of Applied Entomology,2013,50(1):19-28.
[17] 潘程莹，胡婧，张霞，等.斑腿蝗科Catantopidae七种蝗虫线粒体COI基因的DNA条形码研究[J].昆虫分类学报，2006,28(2):103-110.PAN C Y,JING H U,ZHANG X,et al.The DNA barcoding application of mtDNA COI genes in seven species of catantopidae (orthoptera)[J].Entomotaxonomia,2006,28(2):103-110.
[18] 王哲，景若芸，乔格侠.基于DNA条形码对桃属植物上蚜虫的快速鉴定[J].应用昆虫学报，2013,50(1):41-49.WANG Z,JING R R,QIAO K X.Rapid identification of aphids on Amygdalus plants using DNA barcoding[J].Chinese Journal of Aplied Entomology,2013,50(1):41-49.
[19] 乔玮娜，万方浩，张爱兵，等.DNA条形码技术在田间常见蓟马种类识别中的应用[J].昆虫学报，2012,55(3):344-356.QIAO W N,WAN F H,ZHANG A B,et al.Application of DNA barcoding technology for species identification of common thrips (Insecta:Thysanoptera) in China[J].Acta Entomologica Sinica,2012,55(3):344-356.
[20] 汪珍春，邓其芬，陈诗颖，等.DNA条形码快速鉴定广州木槿蚜虫[J].环境昆虫学报，2015,37(3):583-590.WANG,Z C,DENG Q F,CHEN S Y,et al.Rapid identification of aphids on Hibiscus syriacus Linn.in Guangzhou based on DNA barcoding[J].Journal of Environmental Entomology,2015,37(3):583-590.
[21] 汪珍春，王小兰，郑毅胜，等.DNA条形码快速鉴定广州常见菊花蚜虫[J].环境昆虫学报，2013,35(6):764-771.WANG,Z C,WANG X L,ZHENG Y S,et al.Rapid identification of aphids on Chrysanthemum plants in Guangzhou based on DNA barcoding[J].Journal of Environmental Entomology,2013,35(6):764-771.
[22] 郭凤娟，阳建春，胡诗佳，等.DNA条形码在紫斑谷螟幼虫鉴定中的应用[J].环境昆虫学报，2015,37(1):51-55.GUO F J,YANG J C,HU S J,et al.Application of DNA barcoding in Pyralis farinalis larvae[J].Journal of Environmental Entomology,2015,37(1),51-55.
[23] 刘慎思，张桂芬，武强，等.桔小实蝇幼体及成虫残体DNA条形码识别技术的建立与应用[J].昆虫学报，2012,55(3):336-343.LIU S S,ZHANG G F,WU Q,et al.Establishment and application of DNA barcoding technology for identification of the immatures and adult debris of Bactrocera dorsalis (Hendel) (Diptera:Tephritidae)[J].Acta Entomologica Sinica,2012,55(3):336-343.
[24] ZAJEC E.Chromas[J].The Visual Computer,1986,2(3):182-183.
[25] ALZOHAIRY A M.BioEdit:An important software for molecular biology[J].GERF Bulletin of Biosciences,2011,2(1):60-61.
[26] LARKIN M,BLACKSHIELDS G,BROWN N,et al.ClustalW and ClustalX ver.2.0[J].Bioinformatics,2007,23(21):2947-2948.
[27] KOICHIRO T,GLEN S,SUDHIR K.MEGA11:Molecular evolutionary genetics analysis version 11[J].Molecular Biology and Evolution,2021,38(7):3022-3027.
[28] JANZEN D H,HAJIBABAEI M,BURNS J M,et al.Wedding biodiversity inventory of a large and complex Lepidoptera fauna with DNA barcoding[J].Philosophical Transactions of the Royal Society of London,2005,360(1462):1835-1845.
[29] HEBERT P D.RATNASINGHAM S,DE WAARD J R.Barcoding animal life:Cytochrome c oxidase subunit 1 divergences among closely related species.Proceedings of the royal society of London[J].Series B:Biological Sciences,2003,270:96-99.
[30] HEBERT P D,STOECKLE M Y,ZEMLAK T S.Identification of birds through DNA barcodes[J].PLoS Biology,2004,2(10):1657-1663.
[31] MEIER R,SHIYANG K,VAIDYA G,et al.DNA barcoding and taxonomy in Diptera:A tale of high intraspecific variability and low identification success[J].Systematic Biology,2006,55(5):715-728.
[2] ZHONG L K,LI H X,YU X F,et al.Complete mitochondrial genome sequence of Cicadella viridis (Hemiptera:Cicadellidae:Cicadellinae)[J].Mitochondrial DNA Part B,2019,4(1):1287-1288.
[3] ZHANG H G,HE B B,XING J,et al.Spatial and temporal patterns of rice planthopper populations in South and Southwest China[J].Computers and Electronics in Agriculture,2022,194:106750(1-12).
[4] ARDESTANI M M.A brief overview of the rice pests and their natural enemies in some rice-growing Asian countries[J].Russian Agricultural Sciences,2020,46(4):359-364.
[5] SCHINDEL D E,MILLER S E.DNA barcoding a useful tool for taxonomists[J].Nature,2005,435:1-17.
[6] BOLSHAKOV V V,PROKIN A A.Karyotype and COI sequences of Chironomus sokolove istomina,Kiknadze et Siirin,1999 (Diptera,Chironomidae) from the bay of Orkhon River,Mongolia[J].Comparative Cytogenetics,2021,15(2):149-157.
[7] SANDRA M V,ELKIN A,JENNY J G,et al.Saving DNA from museum specimens:The success of DNA mini-barcodes in haplotype reconstruction in the genus Anastrepha (Diptera:Tephritidae)[J].Journal of Advanced Research,2019,16:123-134.
[8] LIN X L,MO L D,BU W J,et al.The first comprehensive DNA barcode reference library of Chinese Tanytarsus (Diptera:Chironomidae) for environmental DNA metabarcoding[J].Diversity and Distributions,2021,27(10):1932-1941.
[9] KRESS W J,ERICKSON D L.DNA barcodes:Genes,genomics,and bioinformatics[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(8):2761-2762.
[10] TAANMAN J W.The mitochondrial genome;structure,transcription,translation and replication[J].Biochimica et Biophysica Acta (BBA)-Bioenergetics,1999,1410(2):103-123.
[11] HEBERT P D,RATNASINGHAM S,DEWAARD J R.Barcoding animal life:Cytochrome c oxidase subunit 1 divergences among closely related species[J].Proceedings of the Royal Society of London.Series B:Biological Sciences,2003,270(suppl 1):96-99.
[12] 刘勇，宋毓，李晓宇.基于线粒体COI基因的DNA条形码技术在昆虫分子鉴定中的应用[J].植物检疫，2010,24(2):46-50.LIU Y,SONG Y,LI X Y.Application of DNA barcoding technology based on the mitochondrial COI gene for molecular identification of insects[J].Plant Quarantine,2010,24(2):46-50.
[13] 李青青，李地艳，段焰青，等.DNA条形码在鳞翅目昆虫中的应用[J].生命科学，2010,22(4):307-312.LI Q Q,LI D Y,DUAN Y Q,et al.Application of DNA barcoding in Lepidopteran insects[J].Chinese Bulletin of Life Sciences,2010,22(4):307-312.
[14] 李晓叶，钱路，陈茂华.DNA条形码技术在几种苹果园鳞翅目害虫鉴定中的应用[J].西北农业学报，2015,24(5):141-147.LI X X,QIAN L,CHENG M H.Application of DNA barcoding technology to identification of some Lepidoptera pests in apple orchards[J].Acta Agriculturae Boreali-occidentalis Sinica,2015,24(5):141-147.
[15] 温娟，陈睿，姜立云，等.基于DNA条形码对北京地区蔷薇科花卉上蚜虫的快速鉴定[J].应用昆虫学报，2013,50(1):29-40.WEN J,CHEN R,JIANG L Y,et al.Rapid identification of aphids on flowering plants of Rosaceae in Beijing based on DNA barcoding[J].Chinese Journal of Applied Entomology,2013,50(1):29-40.
[16] 周青松，张同心，于芳，等.DNA条形码在膜翅目昆虫中的应用分析[J].应用昆虫学报，2013,50(1):19-28.ZHOU Q S,ZHANG T X,YU F,et al.Application of DNA barcoding to Hymenopteran taxonomy[J].Chinese Journal of Applied Entomology,2013,50(1):19-28.
[17] 潘程莹，胡婧，张霞，等.斑腿蝗科Catantopidae七种蝗虫线粒体COI基因的DNA条形码研究[J].昆虫分类学报，2006,28(2):103-110.PAN C Y,JING H U,ZHANG X,et al.The DNA barcoding application of mtDNA COI genes in seven species of catantopidae (orthoptera)[J].Entomotaxonomia,2006,28(2):103-110.
[18] 王哲，景若芸，乔格侠.基于DNA条形码对桃属植物上蚜虫的快速鉴定[J].应用昆虫学报，2013,50(1):41-49.WANG Z,JING R R,QIAO K X.Rapid identification of aphids on Amygdalus plants using DNA barcoding[J].Chinese Journal of Aplied Entomology,2013,50(1):41-49.
[19] 乔玮娜，万方浩，张爱兵，等.DNA条形码技术在田间常见蓟马种类识别中的应用[J].昆虫学报，2012,55(3):344-356.QIAO W N,WAN F H,ZHANG A B,et al.Application of DNA barcoding technology for species identification of common thrips (Insecta:Thysanoptera) in China[J].Acta Entomologica Sinica,2012,55(3):344-356.
[20] 汪珍春，邓其芬，陈诗颖，等.DNA条形码快速鉴定广州木槿蚜虫[J].环境昆虫学报，2015,37(3):583-590.WANG,Z C,DENG Q F,CHEN S Y,et al.Rapid identification of aphids on Hibiscus syriacus Linn.in Guangzhou based on DNA barcoding[J].Journal of Environmental Entomology,2015,37(3):583-590.
[21] 汪珍春，王小兰，郑毅胜，等.DNA条形码快速鉴定广州常见菊花蚜虫[J].环境昆虫学报，2013,35(6):764-771.WANG,Z C,WANG X L,ZHENG Y S,et al.Rapid identification of aphids on Chrysanthemum plants in Guangzhou based on DNA barcoding[J].Journal of Environmental Entomology,2013,35(6):764-771.
[22] 郭凤娟，阳建春，胡诗佳，等.DNA条形码在紫斑谷螟幼虫鉴定中的应用[J].环境昆虫学报，2015,37(1):51-55.GUO F J,YANG J C,HU S J,et al.Application of DNA barcoding in Pyralis farinalis larvae[J].Journal of Environmental Entomology,2015,37(1),51-55.
[23] 刘慎思，张桂芬，武强，等.桔小实蝇幼体及成虫残体DNA条形码识别技术的建立与应用[J].昆虫学报，2012,55(3):336-343.LIU S S,ZHANG G F,WU Q,et al.Establishment and application of DNA barcoding technology for identification of the immatures and adult debris of Bactrocera dorsalis (Hendel) (Diptera:Tephritidae)[J].Acta Entomologica Sinica,2012,55(3):336-343.
[24] ZAJEC E.Chromas[J].The Visual Computer,1986,2(3):182-183.
[25] ALZOHAIRY A M.BioEdit:An important software for molecular biology[J].GERF Bulletin of Biosciences,2011,2(1):60-61.
[26] LARKIN M,BLACKSHIELDS G,BROWN N,et al.ClustalW and ClustalX ver.2.0[J].Bioinformatics,2007,23(21):2947-2948.
[27] KOICHIRO T,GLEN S,SUDHIR K.MEGA11:Molecular evolutionary genetics analysis version 11[J].Molecular Biology and Evolution,2021,38(7):3022-3027.
[28] JANZEN D H,HAJIBABAEI M,BURNS J M,et al.Wedding biodiversity inventory of a large and complex Lepidoptera fauna with DNA barcoding[J].Philosophical Transactions of the Royal Society of London,2005,360(1462):1835-1845.
[29] HEBERT P D.RATNASINGHAM S,DE WAARD J R.Barcoding animal life:Cytochrome c oxidase subunit 1 divergences among closely related species.Proceedings of the royal society of London[J].Series B:Biological Sciences,2003,270:96-99.
[30] HEBERT P D,STOECKLE M Y,ZEMLAK T S.Identification of birds through DNA barcodes[J].PLoS Biology,2004,2(10):1657-1663.
[31] MEIER R,SHIYANG K,VAIDYA G,et al.DNA barcoding and taxonomy in Diptera:A tale of high intraspecific variability and low identification success[J].Systematic Biology,2006,55(5):715-728.
基本信息:
DOI：
中图分类号:S435.112
引用信息:
[1]程依情,邵珠龙,王正亮等.基于DNA条形码的稻蓟马、稻叶蝉和稻飞虱种类鉴别[J].中国计量大学学报,2023,34(01):44-50.
基金信息:
国家自然科学基金项目(NO.31972347,U21A20223);; 浙江省属高校基本科研业务费专项项目(NO.2022YW82)
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