2023 03 v.34;No.111 348-356+464
基于不同配体的NSE检测方法研究进展
基金项目(Foundation):
第七届青托工程项目(No.2021QNRC001)
邮箱(Email):
yxp@cjlu.edu.cn;
DOI:
中文作者单位:
中国计量大学生命科学学院;中国计量科学研究院;长春市计量检定测试技术研究院;
摘要(Abstract):
目的:通过综述神经特异性烯醇化酶(neuron-specific enolase, NSE)检测方法研究进展，为NSE灵敏准确定量检测方法开发提供有力解决方案。方法:结合文献和数据库搜索，总结NSE的结构特征与基本性质，系统介绍近年来基于不同配体的NSE检测方法与技术的研究进展。结果:作为目前最可靠的小细胞肺癌诊断标志物，NSE已被应用于开发多种检测方法与技术，并被应用于临床中肺癌的诊断、预后及疗效评估。结论:目前尚无统一的金标准方法来检测NSE,但已经开发出了多种基于不同配体的检测方法，它们可以用于恶性肿瘤等重大疾病的早期诊断、病情监控和预后评估，为临床提供技术支持。
关键词(KeyWords):
神经元特异性烯醇化酶;;小细胞肺癌;;肿瘤标志物;;临床诊断;;体外检测
| 106 | 0 | 21 |
| 下载次数 | 被引频次 | 阅读次数 |
参考文献
[1] ISGRò M A,BOTTONI P,SCATENA R.Neuron-specific enolase as a biomarker:Biochemical and clinical aspects[J].Advances in Cancer Biomarkers,2015,867:125-143.
[2] MCALEESE S M,DUNBAR B,FOTHERGILL J E,et al.Complete amino acid sequence of the neurone-specific γ isozyme of enolase (NSE) from human brain and comparison with the non-neuronal α form (NNE)[J].European Journal of Biochemistry,1988,178(2):413-417.
[3] GIALLONGO A,FEO S,MOORE R,et al.Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase[J].Proceedings of the National Academy of Sciences,1986,83(18):6741-6745.
[4] CHAI G,BREWER J M,LOVELACE L L,et al.Expression,purification and the 1.8 ? resolution crystal structure of human neuron specific enolase[J].Journal of Molecular Biology,2004,341(4):1015-1021.
[5] CHEN S H,OMENN G S.Human neuron-specific enolase:Genetic and developmental studies[J].Journal of Neurogenetics,1984,1(2):159-164.
[6] NOBELS F R E,KWEKKEBOOM D J,COOPMANS W,et al.Chromogranin A as serum marker for neuroendocrine neoplasia:Comparison with neuron-specific enolase and the α-subunit of glycoprotein hormones[J].The Journal of Clinical Endocrinology & Metabolism,1997,82(8):2622-2628.
[7] ERIKSSON B,?BERG K,STRIDSBERG M.Tumor markers in neuroendocrine tumors[J].Digestion,2000,62(suppl 1):33-38.
[8] BAUDIN E,GIGLIOTTI A,DUCREUX M,et al.Neuron-specific enolase and chromogranin A as markers of neuroendocrine tumours[J].British Journal of Cancer,1998,78(8):1102-1107.
[9] BAJETTA E,FERRARI L,MARTINETTI A,et al.Chromogranin A,neuron specific enolase,carcinoembryonic antigen,and hydroxyindole acetic acid evaluation in patients with neuroendocrine tumors[J].Cancer:Interdisciplinary International Journal of the American Cancer Society,1999,86(5):858-865.
[10] DU J,LI Y,WANG L,et al.Selective application of neuroendocrine markers in the diagnosis and treatment of small cell lung cancer[J].Clinica Chimica Acta,2020,509:295-303.
[11] VAN DELFT F A,SCHUURBIERS M,MULLER M,et al.Modeling strategies to analyse longitudinal biomarker data:An illustration on predicting immunotherapy non-response in non-small cell lung cancer[J].Heliyon,2022,8(10):e10932(1-8).
[12] WU Z Q,LI Y,SUN N N,et al.Nomogram for preoperative estimation of histologic grade in gastrointestinal neuroendocrine tumors[J].Frontiers in Endocrinology,2022,13:991773(1-10).
[13] ZELTZER P M,MARANGOS P J,EVANS A E,et al.Serum neuron-specific enolase in children with neuroblastoma.Crelationship to stage and disease course[J].Cancer,1986,57(6):1230-1234.
[14] SUN T,TANG J,PAN Y C,et al.Serum markers change for intraocular metastasis in renal cell carcinoma[J].Bioscience Reports,2021,41(9):BSR20203116(1-9).
[15] SABIR RASHID A,HUANG-LINK Y,JOHNSSON M,et al.Predictors of early neurological deterioration and functional outcome in acute ischemic stroke:The importance of large artery disease,hyperglycemia and inflammatory blood biomarkers[J].Neuropsychiatric Disease and Treatment,2022,18:1993-2002.
[16] YUN G S,IN Y N,KANG C,et al.Development of a strategy for assessing blood-brain barrier disruption using serum S100 calcium-binding protein B and neuron-specific enolase in early stage of neuroemergencies:A preliminary study[J].Medicine,2022,101(28):e29644(1-6).
[17] MAO S,HUANG H,CHEN X.lncRNA H19 aggravates brain injury in rats following experimental intracerebral hemorrhage via NF-κB pathway[J].Computational and Mathematical Methods in Medicine,2022:3017312(1-7).
[18] FRIIS T,WIKSTR?M A K,ACURIO J,et al.Cerebral biomarkers and blood-brain barrier integrity in preeclampsia[J].Cells,2022,11(5):789(1-14).
[19] LAGEBRANT A,LANG M,NIELSEN N,et al.Brain injury markers in blood predict signs of hypoxic ischaemic encephalopathy on head computed tomography after cardiac arrest[J].Resuscitation,2022,184:109668(1-7).
[20] KOSKENSALO K,VIRTANEN S,SAUNAVAARA J,et al.Comparison of the prognostic value of early-phase proton magnetic resonance spectroscopy and diffusion tensor imaging with serum neuron-specific enolase at 72 h in comatose survivors of out-of-hospital cardiac arrest:A substudy of the XeHypotheca trial[J].Neuroradiology,2023,65(2):349-360.
[21] RICHTER S,WINZECK S,CZEITER E,et al.Serum biomarkers identify critically ill traumatic brain injury patients for MRI[J].Critical Care,2022,26(1):369(1-7).
[22] CHEN W,WANG G,YAO C,et al.The ratio of serum neuron-specific enolase level to admission glasgow coma scale score is associated with diffuse axonal injury in patients with moderate to severe traumatic brain injury[J].Frontiers in Neurology,2022,13:887818(1-8).
[23] GAO Y,DUAN J,JI H,et al.Levels of S100 calcium binding protein B (S100B),neuron-specific enolase (NSE),and cyclophilin A (CypA) in the serum of patients with severe craniocerebral injury and multiple injuries combined with delirium transferred from the ICU and their prognostic value[J].Annals of Palliative Medicine,2021,10:3371-3378.
[24] MCKITTERICK N.Molecularly Imprinted Polymers for the Analysis of Small Cell Lung Cancer Biomarkers by Liquid Chromatography-Tandem Mass Spectrometry[D].Oslo:Universitetet i Oslo,2021:13-21.
[25] ZHONG X,QIAO L,STAUFFER G,et al.On-chip spyhole nanoelectrospray ionization mass spectrometry for sensitive biomarker detection in small volumes[J].Journal of the American Society for Mass Spectrometry,2018,29(7):1538-1545.
[26] KO J A,LIM H B.Metal-doped inorganic nanoparticles for multiplex detection of biomarkers by a sandwich-type ICP-MS immunoassay[J].Analytica Chimica Acta,2016,938:1-6.
[27] TORSETNES S B,LEVERN?S M S,BROUGHTON M N,et al.Multiplexing determination of small cell lung cancer biomarkers and their isovariants in serum by immunocapture LC-MS/MS[J].Analytical Chemistry,2014,86(14):6983-6992.
[28] WEVERS R A,JACOBS A A C,HOMMES O R.A bioluminescent assay for enolase (EC 4.2.1.11) activity in human serum and cerebrospinal fluid[J].Clinica Chimica Acta,1983,135(2):159-168.
[29] TIGHE P J,RYDER R R,TODD I,et al.ELISA in the multiplex era:Potentials and pitfalls[J].PROTEOMICS-Clinical Applications,2015,9(3-4):406-422.
[30] SCHMECHEL D,MARANGOS P J,ZIS A P,et al.Brain enolases as specific markers of neuronal and glial cells[J].Science,1978,199(4326):313-315.
[31] MARANGOS P J,ZOMZELY-NEURATH C,York C.Immunological studies of a nerve specific protein[J].Archives of Biochemistry and Biophysics,1975,170:289-293.
[32] PARMA A M,MARANGOS P J,GOODWIN F K.A more sensitive radioimmunoassay for neuron-specific enolase suitable for cerebrospinal fluid determinations[J].Journal of Neurochemistry,1981,36(3):1093-1096.
[33] PAUS E,NUSTAD K.Immunoradiometric assay for alpha gamma-and gamma gamma-enolase (neuron-specific enolase),with use of monoclonal antibodies and magnetizable polymer particles[J].Clinical Chemistry,1989,35(10):2034-2038.
[34] ENGVALL E,PERLMANN P.Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G[J].Immunochemistry,1971,8(9):871-874.
[35] DUNCAN M E,MCALEESE S M,BOOTH N A,et al.A simple enzyme-linked immunosorbent assay (ELISA) for the neuron-specific γ isozyme of human enolase (NSE) using monoclonal antibodies raised against synthetic peptides corresponding to isozyme sequence differences[J].Journal of Immunological Methods,1992,151(1-2):227-236.
[36] LEQUIN R M.Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA)[J].Clinical Chemistry,2005,51(12):2415-2418.
[37] DIACONU I,CRISTEA C,H?RCEAG? V,et al.Electrochemical immunosensors in breast and ovarian cancer[J].Clinica Chimica Acta,2013,425:128-138.
[38] LI G Z,TIAN F.Guanine-decorated graphene nanostructures for sensitive monitoring of neuron-specific enolase based on an enzyme-free electrocatalytic reaction[J].Analytical Sciences,2013,29(12):1195-1201.
[39] HUANG X,MIAO J,FANG J,et al.Ratiometric electrochemical immunosensor based on L-cysteine grafted ferrocene for detection of neuron specific enolase[J].Talanta,2022,239:123075(1-8).
[40] ZHANG C,MA Z.PtCu nanoprobe-initiated cascade reaction modulated iodide-responsive sensing interface for improved electrochemical immunosensor of neuron-specific enolase[J].Biosensors and Bioelectronics,2019,143:111612(1-6).
[41] FANG Y,LI Y,ZHANG M,et al.A novel electrochemical strategy based on porous 3D graphene-starch architecture and silver deposition for ultrasensitive detection of neuron-specific enolase[J].Analyst,2019,144(6):2186-2194.
[42] SOOMRO R A,KALWAR N H,AVCI A,et al.In-situ growth of NiWO4 saw-blade-like nanostructures and their application in photo-electrochemical (PEC) immunosensor system designed for the detection of neuron-specific enolase[J].Biosensors and Bioelectronics,2019,141:111331(1-7).
[43] LIU R,WANG Y,WONG W,et al.Photoelectrochemical immunoassay platform based on MoS2 nanosheets integrated with gold nanostars for neuron-specific enolase assay[J].Microchimica Acta,2020,187(8):1-9.
[44] ZHANG Q,LI X,QIAN C,et al.Label-free electrochemical immunoassay for neuron specific enolase based on 3D macroporous reduced graphene oxide/polyaniline film[J].Analytical Biochemistry,2018,540:1-8.
[45] AYDIN E B,AYDIN M,SEZGINTüRK M K.Selective and ultrasensitive electrochemical immunosensing of NSE cancer biomarker in human serum using epoxy-substituted poly (pyrrole) polymer modified disposable ITO electrode[J].Sensors and Actuators B:Chemical,2020,306:127613(1-12).
[46] MAHMOUDI T,DE LA GUARDIA M,SHIRDEL B,et al.Recent advancements in structural improvements of lateral flow assays towards point-of-care testing[J].TrAC Trends in Analytical Chemistry,2019,116:13-30.
[47] MOULAHOUM H,GHORBANIZAMANI F,TIMUR S.Based lateral flow assay using rhodamine B-loaded polymersomes for the colorimetric determination of synthetic cannabinoids in saliva[J].Microchimica Acta,2021,188(11):1-12.
[48] DUAN X,SHI Y,ZHANG X,et al.Dual-detection fluorescent immunochromatographic assay for quantitative detection of SARS-CoV-2 spike RBD-ACE2 blocking neutralizing antibody[J].Biosensors and Bioelectronics,2022,199:113883(1-8).
[49] XIAO K,WANG K,QIN W,et al.Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen[J].Talanta,2017,164:463-469.
[50] CAO Z,LI H,LAU C,et al.Cross-talk-free simultaneous fluoroimmunoassay of two biomarkers based on dual-color quantum dots[J].Analytica Chimica Acta,2011,698(1-2):44-50.
[51] MAO Y,WANG N,YU F,et al.Simultaneous detection of carcinoembryonic antigen and neuron-specific enolase in human serum based on time-resolved chemiluminescence immunoassay[J].Analyst,2019,144(16):4813-4819.
[52] MULEY T,EBERT W,STIEBER P,et al.Technical performance and diagnostic utility of the new elecsys?neuron-specific enolase enzyme immunoassay[J].Clinical Chemistry and Laboratory Medicine,2003,41(1):95-103.
[53] YANG L,WU T,DU Y,et al.PEGylation improved electrochemiluminescence supramolecular assembly of iridium (III) complexes in apoferritin for immunoassays using 2D/2D MXene/TiO2 hybrids as signal amplifiers[J].Analytical Chemistry,2021,93(50):16906-16914.
[54] AYDIN E B,AYDIN M,SEZGINTüRK M K.Selective and ultrasensitive electrochemical immunosensing of NSE cancer biomarker in human serum using epoxy-substituted poly (pyrrole) polymer modified disposable ITO electrode[J].Sensors and Actuators B:Chemical,2020,306:127613(1-12).
[55] ZHENG X,MO G,HE Y,et al.Electrochemiluminescent immunoassay for neuron specific enolase by using amino-modified reduced graphene oxide loaded with N-doped carbon quantum dots[J].Microchimica Acta,2019,186(12):1-11.
[56] WANG Y,ZHAO P,MAO L,et al.Determination of brain injury biomarkers by surface-enhanced Raman scattering using hollow gold nanospheres[J].RSC Advances,2018,8(6):3143-3150.
[57] SONG Y,SUN J,ZhAO S,et al.Based lateral flow immunosensor for ultrasensitive and selective surface-enhanced Raman spectroscopy stroke biomarkers detection[J].Applied Surface Science,2022,571:151153(1-12).
[58] WANG W,LI Y,NIE A,et al.A portable SERS reader coupled with catalytic hairpin assembly for sensitive microRNA-21 lateral flow sensing[J].Analyst,2021,146(3):848-854.
[59] 李平华，郭振建，张雅丽.微纳光纤免疫传感器的研制[J].工业设计，2012,68(3):269-269.LI H P,GUO Z J,ZHANG T L.Development of micro/nano fiber optic immunosensor[J].Industrial Design,2012,68(3):269-269.
[60] ZHOU L,LIU C,SUN Z,et al.Black phosphorus based fiber optic biosensor for ultrasensitive cancer diagnosis[J].Biosensors and Bioelectronics,2019,137:140-147.
[61] 石胜辉，王鑫，罗彬彬，等.氧化石墨烯修饰腐蚀型长周期光纤光栅的禽流感病毒免疫传感器[J].光子学报，2020,49(1):51-59.SHI S H,WANG X,LUO B B,et al.Avian Influenza virus immunosensor based on etched long period fiber grating coated with graphene oxide[J].Acta Photonica Sinica,2020,49(1):51-59.
[62] WANG Y,HAN M,LIU G,et al.Molecularly imprinted electrochemical sensing interface based on in-situ-polymerization of amino-functionalized ionic liquid for specific recognition of bovine serum albumin[J].Biosensors and Bioelectronics,2015,74:792-798.
[63] ANDERSSON H S,KOCH-SCHMIDT A C,OHLSON S,et al.Study of the nature of recognition in molecularly imprinted polymers[J].Journal of Molecular Recognition,1996,9(5-6):675-682.
[64] CHEN L,XU S,LI J.Recent advances in molecular imprinting technology:Current status,challenges and highlighted applications[J].Chemical Society Reviews,2011,40(5):2922-2942.
[65] WANG X,WANG Y,YE X,et al.Sensing platform for neuron specific enolase based on molecularly imprinted polymerized ionic liquids in between gold nanoarrays[J].Biosensors and Bioelectronics,2018,99:34-39.
[66] RACHKOV A,MINOURA N.Towards molecularly imprinted polymers selective to peptides and proteins.The epitope approach[J].Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology,2001,1544(1-2):255-266.
[67] PIRZADA M,SEHIT E,ALTINTAS Z.Cancer biomarker detection in human serum samples using nanoparticle decorated epitope-mediated hybrid MIP[J].Biosensors and Bioelectronics,2020,166:112464(1-11).
[68] XING R,WEN Y,DONG Y,et al.Dual molecularly imprinted polymer-based plasmonic immunosandwich assay for the specific and sensitive detection of protein biomarkers[J].Analytical Chemistry,2019,91(15):9993-10000.
[69] ZHAO J,MA Y,HOU X,et al.A novel ionic liquid synthesis,electrochemical polymerization,and sensing performance toward bisphenol A[J].Journal of Solid State Electrochemistry,2015,19(6):1571-1578.
[70] BOSSI A,BONINI F,TURNER A P F,et al.Molecularly imprinted polymers for the recognition of proteins:The state of the art[J].Biosensors and Bioelectronics,2007,22(6):1131-1137.
[71] ANDAC M,GALAEV I Y,DENIZLI A.Molecularly imprinted poly (hydroxyethyl methacrylate) based cryogel for albumin depletion from human serum[J].Colloids and Surfaces B:Biointerfaces,2013,109:259-265.
[72] CHEN A,YANG S.Replacing antibodies with aptamers in lateral flow immunoassay[J].Biosensors and Bioelectronics,2015,71:230-242.
[73] WANG Y,LUO J,LIU J,et al.Label-free microfluidic paper-based electrochemical aptasensor for ultrasensitive and simultaneous multiplexed detection of cancer biomarkers[J].Biosensors and Bioelectronics,2019,136:84-90.
[74] ZHENG Y,ZHAO Y,DI Y,et al.In vitro selection of DNA aptamers for the development of chemiluminescence aptasensor for neuron-specific enolase (NSE) detection[J].RSC Advances,2019,9(27):15513-15520.
[75] SUN L,ShEN K,ZHANG J,et al.Aptamer based surface plasma resonance assay for direct detection of neuron specific enolase and progastrin-releasing peptide (31-98)[J].RSC Advances,2021,11(51):32135-32142.
[76] 李伊凡，金尚忠，姜丽，等.金纳米粒子增敏的SPRi技术应用于遗传性血栓疾病的检测[J].中国计量大学学报，2022,33(3):310-316.LI Y F,JIN S Z,JIANG L,et al.AuNPs assisted detection of genetic thrombotic diseases by theSPRi technique[J].Journal of China University of Metrology,2022,33(3):310-316.
[77] ZHI L J,SUN A L,TANG D.In situ amplified photothermal immunoassay for neuron-specific enolase with enhanced sensitivity using Prussian blue nanoparticle-loaded liposomes[J].Analyst,2020,145(12):4164-4172.
[2] MCALEESE S M,DUNBAR B,FOTHERGILL J E,et al.Complete amino acid sequence of the neurone-specific γ isozyme of enolase (NSE) from human brain and comparison with the non-neuronal α form (NNE)[J].European Journal of Biochemistry,1988,178(2):413-417.
[3] GIALLONGO A,FEO S,MOORE R,et al.Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase[J].Proceedings of the National Academy of Sciences,1986,83(18):6741-6745.
[4] CHAI G,BREWER J M,LOVELACE L L,et al.Expression,purification and the 1.8 ? resolution crystal structure of human neuron specific enolase[J].Journal of Molecular Biology,2004,341(4):1015-1021.
[5] CHEN S H,OMENN G S.Human neuron-specific enolase:Genetic and developmental studies[J].Journal of Neurogenetics,1984,1(2):159-164.
[6] NOBELS F R E,KWEKKEBOOM D J,COOPMANS W,et al.Chromogranin A as serum marker for neuroendocrine neoplasia:Comparison with neuron-specific enolase and the α-subunit of glycoprotein hormones[J].The Journal of Clinical Endocrinology & Metabolism,1997,82(8):2622-2628.
[7] ERIKSSON B,?BERG K,STRIDSBERG M.Tumor markers in neuroendocrine tumors[J].Digestion,2000,62(suppl 1):33-38.
[8] BAUDIN E,GIGLIOTTI A,DUCREUX M,et al.Neuron-specific enolase and chromogranin A as markers of neuroendocrine tumours[J].British Journal of Cancer,1998,78(8):1102-1107.
[9] BAJETTA E,FERRARI L,MARTINETTI A,et al.Chromogranin A,neuron specific enolase,carcinoembryonic antigen,and hydroxyindole acetic acid evaluation in patients with neuroendocrine tumors[J].Cancer:Interdisciplinary International Journal of the American Cancer Society,1999,86(5):858-865.
[10] DU J,LI Y,WANG L,et al.Selective application of neuroendocrine markers in the diagnosis and treatment of small cell lung cancer[J].Clinica Chimica Acta,2020,509:295-303.
[11] VAN DELFT F A,SCHUURBIERS M,MULLER M,et al.Modeling strategies to analyse longitudinal biomarker data:An illustration on predicting immunotherapy non-response in non-small cell lung cancer[J].Heliyon,2022,8(10):e10932(1-8).
[12] WU Z Q,LI Y,SUN N N,et al.Nomogram for preoperative estimation of histologic grade in gastrointestinal neuroendocrine tumors[J].Frontiers in Endocrinology,2022,13:991773(1-10).
[13] ZELTZER P M,MARANGOS P J,EVANS A E,et al.Serum neuron-specific enolase in children with neuroblastoma.Crelationship to stage and disease course[J].Cancer,1986,57(6):1230-1234.
[14] SUN T,TANG J,PAN Y C,et al.Serum markers change for intraocular metastasis in renal cell carcinoma[J].Bioscience Reports,2021,41(9):BSR20203116(1-9).
[15] SABIR RASHID A,HUANG-LINK Y,JOHNSSON M,et al.Predictors of early neurological deterioration and functional outcome in acute ischemic stroke:The importance of large artery disease,hyperglycemia and inflammatory blood biomarkers[J].Neuropsychiatric Disease and Treatment,2022,18:1993-2002.
[16] YUN G S,IN Y N,KANG C,et al.Development of a strategy for assessing blood-brain barrier disruption using serum S100 calcium-binding protein B and neuron-specific enolase in early stage of neuroemergencies:A preliminary study[J].Medicine,2022,101(28):e29644(1-6).
[17] MAO S,HUANG H,CHEN X.lncRNA H19 aggravates brain injury in rats following experimental intracerebral hemorrhage via NF-κB pathway[J].Computational and Mathematical Methods in Medicine,2022:3017312(1-7).
[18] FRIIS T,WIKSTR?M A K,ACURIO J,et al.Cerebral biomarkers and blood-brain barrier integrity in preeclampsia[J].Cells,2022,11(5):789(1-14).
[19] LAGEBRANT A,LANG M,NIELSEN N,et al.Brain injury markers in blood predict signs of hypoxic ischaemic encephalopathy on head computed tomography after cardiac arrest[J].Resuscitation,2022,184:109668(1-7).
[20] KOSKENSALO K,VIRTANEN S,SAUNAVAARA J,et al.Comparison of the prognostic value of early-phase proton magnetic resonance spectroscopy and diffusion tensor imaging with serum neuron-specific enolase at 72 h in comatose survivors of out-of-hospital cardiac arrest:A substudy of the XeHypotheca trial[J].Neuroradiology,2023,65(2):349-360.
[21] RICHTER S,WINZECK S,CZEITER E,et al.Serum biomarkers identify critically ill traumatic brain injury patients for MRI[J].Critical Care,2022,26(1):369(1-7).
[22] CHEN W,WANG G,YAO C,et al.The ratio of serum neuron-specific enolase level to admission glasgow coma scale score is associated with diffuse axonal injury in patients with moderate to severe traumatic brain injury[J].Frontiers in Neurology,2022,13:887818(1-8).
[23] GAO Y,DUAN J,JI H,et al.Levels of S100 calcium binding protein B (S100B),neuron-specific enolase (NSE),and cyclophilin A (CypA) in the serum of patients with severe craniocerebral injury and multiple injuries combined with delirium transferred from the ICU and their prognostic value[J].Annals of Palliative Medicine,2021,10:3371-3378.
[24] MCKITTERICK N.Molecularly Imprinted Polymers for the Analysis of Small Cell Lung Cancer Biomarkers by Liquid Chromatography-Tandem Mass Spectrometry[D].Oslo:Universitetet i Oslo,2021:13-21.
[25] ZHONG X,QIAO L,STAUFFER G,et al.On-chip spyhole nanoelectrospray ionization mass spectrometry for sensitive biomarker detection in small volumes[J].Journal of the American Society for Mass Spectrometry,2018,29(7):1538-1545.
[26] KO J A,LIM H B.Metal-doped inorganic nanoparticles for multiplex detection of biomarkers by a sandwich-type ICP-MS immunoassay[J].Analytica Chimica Acta,2016,938:1-6.
[27] TORSETNES S B,LEVERN?S M S,BROUGHTON M N,et al.Multiplexing determination of small cell lung cancer biomarkers and their isovariants in serum by immunocapture LC-MS/MS[J].Analytical Chemistry,2014,86(14):6983-6992.
[28] WEVERS R A,JACOBS A A C,HOMMES O R.A bioluminescent assay for enolase (EC 4.2.1.11) activity in human serum and cerebrospinal fluid[J].Clinica Chimica Acta,1983,135(2):159-168.
[29] TIGHE P J,RYDER R R,TODD I,et al.ELISA in the multiplex era:Potentials and pitfalls[J].PROTEOMICS-Clinical Applications,2015,9(3-4):406-422.
[30] SCHMECHEL D,MARANGOS P J,ZIS A P,et al.Brain enolases as specific markers of neuronal and glial cells[J].Science,1978,199(4326):313-315.
[31] MARANGOS P J,ZOMZELY-NEURATH C,York C.Immunological studies of a nerve specific protein[J].Archives of Biochemistry and Biophysics,1975,170:289-293.
[32] PARMA A M,MARANGOS P J,GOODWIN F K.A more sensitive radioimmunoassay for neuron-specific enolase suitable for cerebrospinal fluid determinations[J].Journal of Neurochemistry,1981,36(3):1093-1096.
[33] PAUS E,NUSTAD K.Immunoradiometric assay for alpha gamma-and gamma gamma-enolase (neuron-specific enolase),with use of monoclonal antibodies and magnetizable polymer particles[J].Clinical Chemistry,1989,35(10):2034-2038.
[34] ENGVALL E,PERLMANN P.Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G[J].Immunochemistry,1971,8(9):871-874.
[35] DUNCAN M E,MCALEESE S M,BOOTH N A,et al.A simple enzyme-linked immunosorbent assay (ELISA) for the neuron-specific γ isozyme of human enolase (NSE) using monoclonal antibodies raised against synthetic peptides corresponding to isozyme sequence differences[J].Journal of Immunological Methods,1992,151(1-2):227-236.
[36] LEQUIN R M.Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA)[J].Clinical Chemistry,2005,51(12):2415-2418.
[37] DIACONU I,CRISTEA C,H?RCEAG? V,et al.Electrochemical immunosensors in breast and ovarian cancer[J].Clinica Chimica Acta,2013,425:128-138.
[38] LI G Z,TIAN F.Guanine-decorated graphene nanostructures for sensitive monitoring of neuron-specific enolase based on an enzyme-free electrocatalytic reaction[J].Analytical Sciences,2013,29(12):1195-1201.
[39] HUANG X,MIAO J,FANG J,et al.Ratiometric electrochemical immunosensor based on L-cysteine grafted ferrocene for detection of neuron specific enolase[J].Talanta,2022,239:123075(1-8).
[40] ZHANG C,MA Z.PtCu nanoprobe-initiated cascade reaction modulated iodide-responsive sensing interface for improved electrochemical immunosensor of neuron-specific enolase[J].Biosensors and Bioelectronics,2019,143:111612(1-6).
[41] FANG Y,LI Y,ZHANG M,et al.A novel electrochemical strategy based on porous 3D graphene-starch architecture and silver deposition for ultrasensitive detection of neuron-specific enolase[J].Analyst,2019,144(6):2186-2194.
[42] SOOMRO R A,KALWAR N H,AVCI A,et al.In-situ growth of NiWO4 saw-blade-like nanostructures and their application in photo-electrochemical (PEC) immunosensor system designed for the detection of neuron-specific enolase[J].Biosensors and Bioelectronics,2019,141:111331(1-7).
[43] LIU R,WANG Y,WONG W,et al.Photoelectrochemical immunoassay platform based on MoS2 nanosheets integrated with gold nanostars for neuron-specific enolase assay[J].Microchimica Acta,2020,187(8):1-9.
[44] ZHANG Q,LI X,QIAN C,et al.Label-free electrochemical immunoassay for neuron specific enolase based on 3D macroporous reduced graphene oxide/polyaniline film[J].Analytical Biochemistry,2018,540:1-8.
[45] AYDIN E B,AYDIN M,SEZGINTüRK M K.Selective and ultrasensitive electrochemical immunosensing of NSE cancer biomarker in human serum using epoxy-substituted poly (pyrrole) polymer modified disposable ITO electrode[J].Sensors and Actuators B:Chemical,2020,306:127613(1-12).
[46] MAHMOUDI T,DE LA GUARDIA M,SHIRDEL B,et al.Recent advancements in structural improvements of lateral flow assays towards point-of-care testing[J].TrAC Trends in Analytical Chemistry,2019,116:13-30.
[47] MOULAHOUM H,GHORBANIZAMANI F,TIMUR S.Based lateral flow assay using rhodamine B-loaded polymersomes for the colorimetric determination of synthetic cannabinoids in saliva[J].Microchimica Acta,2021,188(11):1-12.
[48] DUAN X,SHI Y,ZHANG X,et al.Dual-detection fluorescent immunochromatographic assay for quantitative detection of SARS-CoV-2 spike RBD-ACE2 blocking neutralizing antibody[J].Biosensors and Bioelectronics,2022,199:113883(1-8).
[49] XIAO K,WANG K,QIN W,et al.Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen[J].Talanta,2017,164:463-469.
[50] CAO Z,LI H,LAU C,et al.Cross-talk-free simultaneous fluoroimmunoassay of two biomarkers based on dual-color quantum dots[J].Analytica Chimica Acta,2011,698(1-2):44-50.
[51] MAO Y,WANG N,YU F,et al.Simultaneous detection of carcinoembryonic antigen and neuron-specific enolase in human serum based on time-resolved chemiluminescence immunoassay[J].Analyst,2019,144(16):4813-4819.
[52] MULEY T,EBERT W,STIEBER P,et al.Technical performance and diagnostic utility of the new elecsys?neuron-specific enolase enzyme immunoassay[J].Clinical Chemistry and Laboratory Medicine,2003,41(1):95-103.
[53] YANG L,WU T,DU Y,et al.PEGylation improved electrochemiluminescence supramolecular assembly of iridium (III) complexes in apoferritin for immunoassays using 2D/2D MXene/TiO2 hybrids as signal amplifiers[J].Analytical Chemistry,2021,93(50):16906-16914.
[54] AYDIN E B,AYDIN M,SEZGINTüRK M K.Selective and ultrasensitive electrochemical immunosensing of NSE cancer biomarker in human serum using epoxy-substituted poly (pyrrole) polymer modified disposable ITO electrode[J].Sensors and Actuators B:Chemical,2020,306:127613(1-12).
[55] ZHENG X,MO G,HE Y,et al.Electrochemiluminescent immunoassay for neuron specific enolase by using amino-modified reduced graphene oxide loaded with N-doped carbon quantum dots[J].Microchimica Acta,2019,186(12):1-11.
[56] WANG Y,ZHAO P,MAO L,et al.Determination of brain injury biomarkers by surface-enhanced Raman scattering using hollow gold nanospheres[J].RSC Advances,2018,8(6):3143-3150.
[57] SONG Y,SUN J,ZhAO S,et al.Based lateral flow immunosensor for ultrasensitive and selective surface-enhanced Raman spectroscopy stroke biomarkers detection[J].Applied Surface Science,2022,571:151153(1-12).
[58] WANG W,LI Y,NIE A,et al.A portable SERS reader coupled with catalytic hairpin assembly for sensitive microRNA-21 lateral flow sensing[J].Analyst,2021,146(3):848-854.
[59] 李平华，郭振建，张雅丽.微纳光纤免疫传感器的研制[J].工业设计，2012,68(3):269-269.LI H P,GUO Z J,ZHANG T L.Development of micro/nano fiber optic immunosensor[J].Industrial Design,2012,68(3):269-269.
[60] ZHOU L,LIU C,SUN Z,et al.Black phosphorus based fiber optic biosensor for ultrasensitive cancer diagnosis[J].Biosensors and Bioelectronics,2019,137:140-147.
[61] 石胜辉，王鑫，罗彬彬，等.氧化石墨烯修饰腐蚀型长周期光纤光栅的禽流感病毒免疫传感器[J].光子学报，2020,49(1):51-59.SHI S H,WANG X,LUO B B,et al.Avian Influenza virus immunosensor based on etched long period fiber grating coated with graphene oxide[J].Acta Photonica Sinica,2020,49(1):51-59.
[62] WANG Y,HAN M,LIU G,et al.Molecularly imprinted electrochemical sensing interface based on in-situ-polymerization of amino-functionalized ionic liquid for specific recognition of bovine serum albumin[J].Biosensors and Bioelectronics,2015,74:792-798.
[63] ANDERSSON H S,KOCH-SCHMIDT A C,OHLSON S,et al.Study of the nature of recognition in molecularly imprinted polymers[J].Journal of Molecular Recognition,1996,9(5-6):675-682.
[64] CHEN L,XU S,LI J.Recent advances in molecular imprinting technology:Current status,challenges and highlighted applications[J].Chemical Society Reviews,2011,40(5):2922-2942.
[65] WANG X,WANG Y,YE X,et al.Sensing platform for neuron specific enolase based on molecularly imprinted polymerized ionic liquids in between gold nanoarrays[J].Biosensors and Bioelectronics,2018,99:34-39.
[66] RACHKOV A,MINOURA N.Towards molecularly imprinted polymers selective to peptides and proteins.The epitope approach[J].Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology,2001,1544(1-2):255-266.
[67] PIRZADA M,SEHIT E,ALTINTAS Z.Cancer biomarker detection in human serum samples using nanoparticle decorated epitope-mediated hybrid MIP[J].Biosensors and Bioelectronics,2020,166:112464(1-11).
[68] XING R,WEN Y,DONG Y,et al.Dual molecularly imprinted polymer-based plasmonic immunosandwich assay for the specific and sensitive detection of protein biomarkers[J].Analytical Chemistry,2019,91(15):9993-10000.
[69] ZHAO J,MA Y,HOU X,et al.A novel ionic liquid synthesis,electrochemical polymerization,and sensing performance toward bisphenol A[J].Journal of Solid State Electrochemistry,2015,19(6):1571-1578.
[70] BOSSI A,BONINI F,TURNER A P F,et al.Molecularly imprinted polymers for the recognition of proteins:The state of the art[J].Biosensors and Bioelectronics,2007,22(6):1131-1137.
[71] ANDAC M,GALAEV I Y,DENIZLI A.Molecularly imprinted poly (hydroxyethyl methacrylate) based cryogel for albumin depletion from human serum[J].Colloids and Surfaces B:Biointerfaces,2013,109:259-265.
[72] CHEN A,YANG S.Replacing antibodies with aptamers in lateral flow immunoassay[J].Biosensors and Bioelectronics,2015,71:230-242.
[73] WANG Y,LUO J,LIU J,et al.Label-free microfluidic paper-based electrochemical aptasensor for ultrasensitive and simultaneous multiplexed detection of cancer biomarkers[J].Biosensors and Bioelectronics,2019,136:84-90.
[74] ZHENG Y,ZHAO Y,DI Y,et al.In vitro selection of DNA aptamers for the development of chemiluminescence aptasensor for neuron-specific enolase (NSE) detection[J].RSC Advances,2019,9(27):15513-15520.
[75] SUN L,ShEN K,ZHANG J,et al.Aptamer based surface plasma resonance assay for direct detection of neuron specific enolase and progastrin-releasing peptide (31-98)[J].RSC Advances,2021,11(51):32135-32142.
[76] 李伊凡，金尚忠，姜丽，等.金纳米粒子增敏的SPRi技术应用于遗传性血栓疾病的检测[J].中国计量大学学报，2022,33(3):310-316.LI Y F,JIN S Z,JIANG L,et al.AuNPs assisted detection of genetic thrombotic diseases by theSPRi technique[J].Journal of China University of Metrology,2022,33(3):310-316.
[77] ZHI L J,SUN A L,TANG D.In situ amplified photothermal immunoassay for neuron-specific enolase with enhanced sensitivity using Prussian blue nanoparticle-loaded liposomes[J].Analyst,2020,145(12):4164-4172.
基本信息:
DOI：
中图分类号:R734.2;R446.1
引用信息:
[1]梁展玮,彭涛,胡亚琳等.基于不同配体的NSE检测方法研究进展[J].中国计量大学学报,2023,34(03):348-356+464.
基金信息:
第七届青托工程项目(No.2021QNRC001)
暂无数据