2024 03 v.35 363-373
磷酸化肽段分离富集方法的研究进展
基金项目(Foundation):
国家重点研发计划项目(No.2022YFF0608400);
国家自然科学基金项目(No.21927812);
中国计量科学研究院基本科研业务费项目(No.AKY1955,AKY1941,AKY1942)
邮箱(Email):
yxp@cjlu.edu.cn;zhairui@nim.ac.cn;;
DOI:
中文作者单位:
中国计量大学生命科学学院浙江省生物计量及检验检疫技术重点实验室;中国计量科学研究院前沿计量科学中心国家市场监管技术创新中心(质谱);
摘要(Abstract):
目的:通过综述O-磷酸化肽段和N-磷酸化肽段的富集方法研究进展，为磷酸化蛋白质分析前处理提供解决方案。方法:结合文献和数据库搜索，总结O-磷酸化肽段和N-磷酸化肽段的富集方法基本原理与应用，系统介绍O-磷酸化肽段和N-磷酸化肽段的富集方法研究进展。结果:磷酸化肽段分离富集方法已被广泛应用于磷酸化蛋白质组学研究中。结论:目前已开发了多种针对不同磷酸化肽段的富集方法，不同的方法具有不同的特异性和选择性，因此，根据研究目的选择富集方法尤为重要。
关键词(KeyWords):
磷酸化肽段;磷酸化肽段富集方法;质谱技术
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[39] ALPERT A J,HUDEC O,MECHTLER K.Anion-Exchange chromatography of phosphopeptides:Weak anion exchange versus strong anion exchange and anion-exchange chromatography versus electrostatic repulsion-hydrophilic interaction chromatography[J].Analytical Chemistry,2015,87(9):4704-4711.
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[41] ALPERT A J.Effect of salts on retention in hydrophilic interaction chromatography[J].Journal of Chromatography A,2018,1538:45-53.
[42] CUI Y,TABANG D,ZHANG Z,et al.Counterion optimization dramatically improves selectivity for phosphopeptides and glycopeptides in electrostatic repulsion-hydrophilic interaction chromatography[J].Analytical Chemistry,2021,93(22):7908-7916.
[43] MIJIN J C V D,LABOTS M,PIERSMA S R,et al.Evaluation of different phospho-tyrosine antibodies for label-free phosphoproteomics[J].Journal of Proteomics,2015,127:259-263.
[44] CONRADS T P,VEENSTRA T D.An enriched look at tyrosine phosphorylation[J].Nature Biotechnology,2005,23(1):36-37.
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[46] BOERSEMA P J,FOONG L Y,DING V M Y,et al.In-depth qualitative and quantitative profiling of tyrosine phosphorylation using a combination of phosphopeptide immunoaffinity purification and stable isotope dimethyl labeling[J].Molecular & Cellular Proteomics,2010,9(1):84-99.
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基本信息:
DOI：
中图分类号:Q503
引用信息:
[1]俞晓平,刘媛,翟睿等.磷酸化肽段分离富集方法的研究进展[J].中国计量大学学报,2024,35(03):363-373.
基金信息:
国家重点研发计划项目(No.2022YFF0608400); 国家自然科学基金项目(No.21927812); 中国计量科学研究院基本科研业务费项目(No.AKY1955,AKY1941,AKY1942)